INTERLEUKIN-1 AND PROSTAGLANDIN-E2 LEVELS ASSOCIATED WITH PERIODONTITIS FROM DIAGNOSIS THROUGH MAINTENANCE
Many inflammatory components of gingival crevicular fluid (GCF) have been studied as potential indicators of periodontal disease activity. The purpose of this study was to correlate site specific IL-1 and PGE2 levels in patients undergoing treatment for adult periodontitis. These tests were used to study disease activity longitudinally from diagnosis through periodontal supportive therapy (maintenance). Eleven sites from the most severely affected posterior sextant were selected from 13 subjects. After diagnosis, treatment consisted of oral hygiene instruction, scaling and root planing, re-evaluation, appropriate surgical therapy (either open flap for debridement, resective or regenerative therapy) and 2-3 month maintenance recalls at the completion of all active therapy. Clinical measurements were recorded, and GCF samples were collected from each of the 11 monitored sites at the following times: at diagnosis, 1 month after scaling and root planing, 3 months after surgery, and at the second maintenance visit (7-10 months post-surgery). The clinical measurements evaluated were probing depth, attachment level, and bleeding on probing. GCF was collected on methyl cellulose strips, the volume was estimated with a Periotron 6000®, and the GCF was analyzed for PGE2 and IL-1 by an ELISA technique. Periodontal therapy resulted in significant improvement in clinical measurements. Laboratory analyses indicate that levels of both IL-1 and PGE2 did not decrease significantly until the second maintenance visit in the diseased/surgically treated sites. Healthy/nonsurgically treated sites did not show a reduction in IL-1 and PGE2 at any time point.