DETECTION OF HUMAN PAPILLOMA VIRUS, EPSTEIN BARR VIRUS, AND CYTOMEGALOVIRUS IN SALIVARY GLAND TISSUE FROM SJÖGREN’S SYNDROME PATIENTS BY POLYMERASE CHAIN REACTION
Primary Sjogren’s syndrome presents with the symptoms of dry eyes and dry mouth. Secondary Sjogren’s, in addition, has an associated rheumatologic condition. It is an autoimmune exocrinopathy of unknown etiology, although viruses have been proposed. Fifteen archived samples of minor (labial) salivary gland tissue from histopathologically diagnosed Sjogren’s patients and fifteen archived samples diagnosed as mucocoeles (controls) were examined to evaluate the presence of human papilloma virus (HPV), Epstein Barr virus (EBV), and cytomegalovirus (CMV). All samples were formalin-fixed and embedded in paraffin. DNA was extracted from 50 mm formalin-fixed paraffin embedded tissue sections (Puregene DNA Isolation Kit, Gentra Systems, Inc.) and amplified by polymerase chain reaction techniques (PCR). PCR products were visualized on either polyacrylamide gels using a 373 ABI Automated DNA sequencer or in agarose/ethidium bromide gels. To insure that DNA extracts performed in the PCR, they were amplified for the exon 8 fragment of the P53 gene and for a T-cell receptor gene. Although positive reactions were seen with these markers, no evidence of EBV or CMV DNA was found. Evidence of HPV DNA was found in mucocoeles and Sjorgren’s tissue samples but the results were not significantly different.